During the trip, I
managed to learn about how DNA is sequenced and analysed, namely by the processes of polymerase chain reaction (PCR) and gel electrophoresis respectively. I learnt that PCR involves using DNA polymerase and primers to replicate strands of DNA and mass-produce the specific gene of interest. Subsequently, it is analysed with gel electrophoresis, which works like a chromatography and can separate DNA strands by mass, allowing for easy identification of the gene and also to confirm that the gene of interest has successfully been amplified. I also learnt about the applications of DNA sequencing, such as in targeted drug therapies
I also managed to
learn about how the above is done in a laboratory, and several of the lab processes involved in DNA sequencing. For example, I was made aware of the distinction between the conventional PCR (cPCR) machine and the real-time PCR (RT-PCR) machine. The cPCR machine is smaller, cheaper and more portable, while the RT-PCR gives the added function of being able to provide instantaneous feedback regarding the amplification of the gene. I also learnt how to do a PCR and subsequent gel electrophoresis by myself, as well as the safety precautions in doing so.
For my part, I
found the trip quite enjoyable as well, and I enjoyed discovering that Life Tech had its own Youtube channel. I even memorised one of the songs there before the trip - "Ph. Diva and the Mystery Band" - and was pleasantly surprised to discover that it was relevant to the content I learnt that day. In fact, I was singing the song under my breath for almost the whole duration of the trip, such was my fascination.
This trip was very informative, and I look forward to similar experiences in the
future.
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